What's it?
The main function of pGlyco+ is as follows: (1) automatically search intact glycopeptides by intact glycopeptide-spectrum match scoring; (2) both support identification of N-Glycopeptide and O-Glycopeptide. Its main technical features include: (1) identification of glycopeptide sugar chain by using open search method, and then use a limited search method to identify the de-glycopeptide; (2) According to the Bayesian algorithm, using the EM method to get the false rate of glycopeptide identification.
How to use it?
pGlyco+ enables intact glycopeptide identification by using (1)a strategy of stepped-energy fragmentation in HCD mode (2)a dedicated search engine of pGlyco+ and (3) validation with 15N/13C16 metabolically labeled samples.
Institute
Fudan University (复旦大学)
Author
Wenfeng Zeng (曾文峰), Fan Zhong(钟凡)
Support
Publication
Funding source
[{"id":"1","name":"CNHPP int'l project 3:2014DFB30020(中国人类蛋白质组学数据的知识发现)"}]